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rabbit antiphospho eef2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit antiphospho eef2
    Rabbit Antiphospho Eef2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 451 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antiphospho+eef2/Phospho-eEF2+(Thr56)+Antibody/pm34323596-128-109-113
    Average 96 stars, based on 451 article reviews
    rabbit antiphospho eef2 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: Metabolic activation of glucose low-responsive beta-cells by glyceraldehyde correlates with their biosynthetic activation in lower glucose concentration range but not at high glucose.
    Article Snippet: .. After electrophoresis, protein was electronically transferred to a nitrocellulose membrane and incubated with one of the following antibodies: anti-4E-BP1 (Santa Cruz Biotechnology, Santa Cruz, CA) at 1:200 dilution; antiphospho-eIF2 (Cell Signaling Technology, Beverly, MA; phosphorylation site Ser 51), antiphospho-rS6 (Cell Signaling Technology; phosphorylation site Ser235/236) and antiphospho-eEF2 (Cell Signaling Technology; phosphorylation site Thr 56) all at 1:1000 dilution. .. After stripping of the nitrocellulose membranes, total protein levels were detected using anti-rS6 and anti-eEF2 (both from Cell Signaling Technology) both at 1:1000 dilution and anti-eIF2 (Santa Cruz) at 1:200 dilution.

    Membrane:

    Article Title: Metabolic activation of glucose low-responsive beta-cells by glyceraldehyde correlates with their biosynthetic activation in lower glucose concentration range but not at high glucose.
    Article Snippet: .. After electrophoresis, protein was electronically transferred to a nitrocellulose membrane and incubated with one of the following antibodies: anti-4E-BP1 (Santa Cruz Biotechnology, Santa Cruz, CA) at 1:200 dilution; antiphospho-eIF2 (Cell Signaling Technology, Beverly, MA; phosphorylation site Ser 51), antiphospho-rS6 (Cell Signaling Technology; phosphorylation site Ser235/236) and antiphospho-eEF2 (Cell Signaling Technology; phosphorylation site Thr 56) all at 1:1000 dilution. .. After stripping of the nitrocellulose membranes, total protein levels were detected using anti-rS6 and anti-eEF2 (both from Cell Signaling Technology) both at 1:1000 dilution and anti-eIF2 (Santa Cruz) at 1:200 dilution.

    Incubation:

    Article Title: Metabolic activation of glucose low-responsive beta-cells by glyceraldehyde correlates with their biosynthetic activation in lower glucose concentration range but not at high glucose.
    Article Snippet: .. After electrophoresis, protein was electronically transferred to a nitrocellulose membrane and incubated with one of the following antibodies: anti-4E-BP1 (Santa Cruz Biotechnology, Santa Cruz, CA) at 1:200 dilution; antiphospho-eIF2 (Cell Signaling Technology, Beverly, MA; phosphorylation site Ser 51), antiphospho-rS6 (Cell Signaling Technology; phosphorylation site Ser235/236) and antiphospho-eEF2 (Cell Signaling Technology; phosphorylation site Thr 56) all at 1:1000 dilution. .. After stripping of the nitrocellulose membranes, total protein levels were detected using anti-rS6 and anti-eEF2 (both from Cell Signaling Technology) both at 1:1000 dilution and anti-eIF2 (Santa Cruz) at 1:200 dilution.

    Phospho-proteomics:

    Article Title: Metabolic activation of glucose low-responsive beta-cells by glyceraldehyde correlates with their biosynthetic activation in lower glucose concentration range but not at high glucose.
    Article Snippet: .. After electrophoresis, protein was electronically transferred to a nitrocellulose membrane and incubated with one of the following antibodies: anti-4E-BP1 (Santa Cruz Biotechnology, Santa Cruz, CA) at 1:200 dilution; antiphospho-eIF2 (Cell Signaling Technology, Beverly, MA; phosphorylation site Ser 51), antiphospho-rS6 (Cell Signaling Technology; phosphorylation site Ser235/236) and antiphospho-eEF2 (Cell Signaling Technology; phosphorylation site Thr 56) all at 1:1000 dilution. .. After stripping of the nitrocellulose membranes, total protein levels were detected using anti-rS6 and anti-eEF2 (both from Cell Signaling Technology) both at 1:1000 dilution and anti-eIF2 (Santa Cruz) at 1:200 dilution.



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    Cell Signaling Technology Inc antiphospho thr56 eef2 antibodies
    Fig. 1. <t>eEF2</t> <t>Thr56</t> phosphorylation under normoxic (A) and ischemic (B) conditions in perfused hearts from WT and AMPKα2 KO mice. A representative immunoblot is shown below blot quantification by densitometric scanning of all immunoblots. The values are the means ± S.E.M. of at least 7 hearts. *P b 0.05 vs. WT.
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    Fig. 1. <t>eEF2</t> <t>Thr56</t> phosphorylation under normoxic (A) and ischemic (B) conditions in perfused hearts from WT and AMPKα2 KO mice. A representative immunoblot is shown below blot quantification by densitometric scanning of all immunoblots. The values are the means ± S.E.M. of at least 7 hearts. *P b 0.05 vs. WT.
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    Fig. 1. <t>eEF2</t> <t>Thr56</t> phosphorylation under normoxic (A) and ischemic (B) conditions in perfused hearts from WT and AMPKα2 KO mice. A representative immunoblot is shown below blot quantification by densitometric scanning of all immunoblots. The values are the means ± S.E.M. of at least 7 hearts. *P b 0.05 vs. WT.
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    Fig. 1. <t>eEF2</t> <t>Thr56</t> phosphorylation under normoxic (A) and ischemic (B) conditions in perfused hearts from WT and AMPKα2 KO mice. A representative immunoblot is shown below blot quantification by densitometric scanning of all immunoblots. The values are the means ± S.E.M. of at least 7 hearts. *P b 0.05 vs. WT.
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    Image Search Results


    Fig. 1. eEF2 Thr56 phosphorylation under normoxic (A) and ischemic (B) conditions in perfused hearts from WT and AMPKα2 KO mice. A representative immunoblot is shown below blot quantification by densitometric scanning of all immunoblots. The values are the means ± S.E.M. of at least 7 hearts. *P b 0.05 vs. WT.

    Journal: Biochimica et biophysica acta

    Article Title: Differential regulation of eEF2 and p70S6K by AMPKalpha2 in heart.

    doi: 10.1016/j.bbadis.2013.02.015

    Figure Lengend Snippet: Fig. 1. eEF2 Thr56 phosphorylation under normoxic (A) and ischemic (B) conditions in perfused hearts from WT and AMPKα2 KO mice. A representative immunoblot is shown below blot quantification by densitometric scanning of all immunoblots. The values are the means ± S.E.M. of at least 7 hearts. *P b 0.05 vs. WT.

    Article Snippet: The phosphorylation state of p70S6K, mTOR, Raptor, PKB/Akt, S6 and eEF2 was assessed by immunoblotting using anti-phospho-Thr389 p70S6K, anti-phospho-Ser2448 mTOR, anti-phospho-Ser792 Raptor, anti-phospho-Thr473 PKB/Akt, anti-phospho-Ser235/236 S6 and antiphospho-Thr56 eEF2 antibodies (all from Cell Signaling), respectively.

    Techniques: Phospho-proteomics, Western Blot